Transcription Section of the Central Dogma

Transcription Section of the Central Dogma Fulya TÃÅ"RKER TRANSCRIPTION: ONE OF THE KEY POINTS OF THE CENTRAL DOGMA ALTERNATIVE SPLICING It is known that the central dogma is the most important principle for an organism to carry on its lifespan. In the central dogma, there are 3 major stages: DNA replication and repair, transcription and translation. DNA replication can be simply described as the duplication of DNA. Transcription is the process of conversion DNA to mRNA. Last step is the translation which means the production of polypeptides from the mRNA, it is simply called as protein synthesis. In this essay, transcription section of the central dogma will be explained with detail. Why transcription is essential for living organisms? Without transcription there is no way to express genes. In order to synthesise protein, at first the mRNA should be formed from a DNA template. Proteins are the functional units in the cells which determine the phenotype of the living organisms. Since the polypeptide chains are formed from the mRNA, there should be a mechanism to create mRNA since it is not found in the cells initially. The proteins that are used in the determination of the phenotype carries the information of the used template DNA strand. DNA sequence is converted to mRNA sequence which then calls the amino acids according to this information. The amino acids form peptide bond with each other and at the end create one, long polypeptide chain. In order to initiate the process, there should be opening of two DNA strands. Since DNA is found as the double helix in the cells, when they are tightly bound to each other, RNA polymerase (the enzyme that adds nucleotides in the 3’ end of the newly formed mRNA strands) can’t bind its template and so transcription can’t initiate. So, the cell must find a way to break the hydrogen bonds between the bases of the DNA strands. In the process of transcription, the essential element is an enzyme, RNA polymerase. What is the significance of this enzyme? AT first, it helps DNA strand to open up for a specific place. The strands are separated from each other and one of them will be selected by polymerase in order to bind and use the information. The newly formed RNA will be carrying the complementary base pairs of the strand that it binds and will have the same sequence with the other strand of the DNA. These are really important points. Of course the new strand will be RNA and eventually will carry uracil instead of thymine. Secondly, in order to elongate the RNA strand, there should be addition of ribonucleotides. During DNA replication, since we are creating new DNA strand, DNA polymerase is responsible for addition of deoxyribonucleotide. However, in transcription we are concerning about RNA strand production. That’s why our enzyme should use ribonuclrotides (adenine, guanine, cytosine, thymine). The hydrogen bonds that are formed between the RNA and DNA strands are very unstable. That’s why RNA polymerase only allows very small length of binding sequence. Also, another important question is that: how the energy for RNA polymerase to move is provided? Our ribonucleotides are carrying three phosphate molecules. That’s why, they are called adenosine triphosphate or guanine etc. When they are added to the newly formed strand, they will release two of the phosphate in their bases and provide the energy necessary for RNA polymerase. There are some significant differences between the RNA polymerase and DNA polymerase except that one is taking role in replication the other is the enzyme for transcription. As indicated before, DNA polymerase is taking place in the addition of deoxyribonucleotides in the replicated DNA, while RNA polymerase adds ribonucleotides in the newly transcribed RNA molecule. Also, DNA polymerase needs a primer in order to initiate the transcription, but for RNA polymerase it is not necessary. Lastly, in the replication, the repair mechanism is highly active since we are duplicating the DNA, the errors shouldn’t be tolerated that much, but during the transcription there might be some tolerance to mistakes since after the transcription the deformed or faulty mRNA will be recognized by a mechanism and be degraded. There are five major classes of RNAs. First one is the mRNA (messenger RNA). It is so much important since it is the template and carries the genetic information for the protein synthesis. In the cell, it can be found in really small amounts. The importance of the function and the amount of it is inversely proportional in this case. Second one is the rRNA (ribosomal RNA). This is the most abundant RNA type in the living organisms’ cells. As the name is indicating, this RNA type is responsible for the structural design of the ribosomes. Ribosome (which is the place where the translation occurs) is mostly composed of rRNAs and the ribosomal proteins. Ribosome formed in the nucleolus by the association of the rRNA and ribosomal proteins. Third one is also really important for the protein synthesis to occur, it is the most important adaptor in the living organisms’ cells. It is tRNA (transfer RNA); include anticodons which recognises the codons on the mRNA and then attach t o the corresponding protein and brings the amino acid to the ribosome structure and helps forming of the correctly ordered polypeptide chain. Fourth one is the snRNA that is responsible for the splicing of premature mRNA. The other types of RNAs are providing various benefits to the cell and take role in the cellular activities. It is known that the elongation of the new strand should be from 5’ to 3’. So this newly formed strand should use the template that is moving from 3’ to 5’. By pairing with the 3’ end, its initial point will be 5’ and it will elongate through 3’. According to the direction of the movement of RNA polymerase, the mechanism will decide which strand (bottom or top strand) will be used by looking for the 3’ end of the DNA template. Since there are different kinds of RNAs are formed, there should also be different type of RNA polymerase in eukaryotic cells. RNA polymeraseI is used in the transcription of specific rRNA molecules. RNA polymerase is used in order to produce genes that carries the information for protein synthesis (including mRNA) and also snRNA. Lastly RNA polymerase III transcribes the tRNA molecules, some rRNA and snRNA. The mechanism of the transcription is a little bit complicated. Because there are lots of additional proteins are involved in the process, without them transcription can’t occur in eucaryotes. There will be comparison between prokaryotic and eukaryotic transcription machinery in the following parts of this essay. Let’s start with the transcription of the eukaryotes since it is much more complicated when it is compared to the prokaryotic system. At first, for the initiation of transcription in the eukaryotic gene has a specific sequence which is called as promoter and composed of TATA sequence. This sequence has an essential role in the transcription Ã…Å ¸n order to initiate the process. This TATA sequence is around 25 base pairs upstream from the initiator site and RNA polymerase II recognizes this site and can binds there with the help of protein complex which is called as transcription factors .The first general factor that is used in the process is TFIID and cont ains a specific part that is called TBP (TATA binding protein). TBP will fit the TATA box and activates the addition of other general transcription factor binding. When they are added to the complex RNA polymerase (contains other transcription factor on it) will be able to bind to the start site. Another important factor in the transcription initiation is the activator proteins. Those areTFIIH has a key role in this process since its ability to give the signals for the unwinding of the DNA strands by hydrolysis of the ATP. This ATP usage by TFIIH causes some modification to occur on the RNA polymerase (mostly phosphorylation). This process changes the RNA polymerase’s shape and allows the detachment of the transcription factors from the complex so from now on the initiation of the transcription ends and elongation process is ready to start. For the transcription initiation to carry on, there are some specific sequence on the genome that are called â€Å"enhancers†. Enhancers have a specific property, they are the site for activator protein binding. Enhancers may be thousands of base pair away from the RNA polymerase binding site however it has a specific ability to bend over and find the RNA polymerase so eventually let the activator proteins to interact with the other transcriptional factors on the start site or on the RNA polymerase. In addition to that in order to create a chance for the protein complex to bind to DNA, DNA must be loosely packed. Since, in normal conditions, DNA is found in a very strictly packaged conformation, this must change. Proteins need some sequence to be bound, however if the DNA is packaged strictly in the nucleosome, the transcription factor binding is impossible. So, there are some complex processes that are responsible for the change the packed conformation of the DNA and increase the approachability of the DNA by the transcriptional factor and RNA polymerase. There are mainly two ways to accomplish this aim: chromatin remodelling complex and histone modification. Remodelling complex separates the histones from the DNA strands slightly and the DNA will have a loose conformation. Histone modification is the second way for the increasing of the deforming the packed DNA. Histone acetylation is the best known technique. Histone acetylation causes the histone proteins on the nucleosome t o release the DNA slightly and make protein binding to DNA possible. Second step of the transcription is called the elongation process. There are some elongation factors which provide the attachment of RNA polymerase to DNA throughout the transcription process. Also, they carry out the RNA polymerase and increase its tolerance to the different sequences that should be transcribed. In the elongation stage, the ribonucleotides will be added to the newly formed RNA strand and at the end there will be a termination signal which causes RNA polymerase-DNA interaction breakage and lead to the product which is called precurser mature mRNA (pre-mRNA). Eukaryotic pre-mRNA needs to be exposed to some modification and of course alternative splicing. Since our genome, most of the eukaryotic organisms’ genome is composed of coding (exon) and non-coding (intron) regions, in order to translation process to occur, the pre-mRNA must cleaved from the intron sequences. In addition to that, pre-mRNA needs to be modified and the 2 ends of the pre-mRNA must have some additional feature. This is important because the translational process can’t occur without the cap modifications. Those modifications marks the mRNA as a healthy and usable product and also help the mRNA to be transported to the cytoplasm (protein synthesis occurs in the cytoplasm) from the nucleus. Firstly, let’s indicate the cap modifications. In the pre-mRNA, there are 2 caps: 5’ cap and 3’ cap. 5’ cap should be modified by the addition of 7-methylguanosine. This procedure is activated by phosphates enzyme, guanyl transferase (GTP to GMP+PP) and methyl transferase. By addition of methylguanosine, the mRNA product is separated from the other RNA molecules and also mRNA, now, will be able to transported to the cytoplasm. 3’ cap is also exposed to additional modification: Poly Adenine tail. At the end of the mRNA product, there will be addition of adenine ribonucleotides and this sequence will prevent the degradation of the mRNA. However, the most exciting and different process that the eukaryotic pre-mRNA is faced with is the splicing. At the beginning and end of each intron, there are a specific sequences that indicates that the machinery is dealing with an intron. The 5’ end of the intron mostly contains GU and 3’ end of it contains AG. Also we have specific base in the middle of the intron, Adenine, which is also called as a branch point and gives the signal for 5’ end binding and the formation of the lariat with the help of the snRNPs. What are the snRNPs and what is their role? At first, as it is claimed in the earliest pages of this essay, there is a specific RNA type which is known as snRNA (small nuclear RNA) which are the important factors in the RNA splicing. In the splicing theory, the 5 of them play an active role: U1, U2, U4, U5, U6. Those particles recognise the exon and intron end and start points and can distinguish them so help the splicing process a lot. Each of them co ntain at least seven proteins and form snRNPs (small nuclear ribonucleoprotein) which afterwards creates a structure that is called a â€Å"spliceosome†. Now, in the following section each snRNP that is actively join the splicing process will be explained. At first, there is a BBP protein which binds to the branch point (mostly Adenine). U2 snRNP recognizes BBP binding and replaces this protein with itself and form interaction with the branch point. U2 pushes A to the outside of the sequence and allows the attack of the 5’ site to this specific base. U1 initially recognises the 5’ end of the intron. U4-U5-U6 joins the process as a triplet. U4 and U6 is dissociated from each other and U6 removes the U1 snRNP and sits onto the 5’ end. U6 and branch point interacts and come closer (the process is called first phosphoryl transfer-reaction). At the end of this process lariat formation occurs. Lastly, U5 causes exon-exon interactions and second phosphoryl-transfer reaction takes place. At the end of this process the RNA is spliced and the mature mRNA is formed. In mature mRNA, there is 5’ cap, 3’ poly a tail and no introns. From now on, this mRNA is ready to synthesise the protein (the process of translatio n). Also, there is a theory of alternative splicing which should be mentioned in the discussion about the RNA splicing subject. After the removal of the introns, some exon can leave the sequence and cause alternative sequences. In this process, no shuffling of exons is allowed, but some of them leave the track. The first exon, which carries the start codon AUG, can’t change. It must be always found in the first position of the mature mRNA. The last exon of the spliced RNA must also contain one of the stop codons (UAA,UAG, UGA). But, other exon sequences are allowed to change without shuffling. Exon orders must be preserved. The below diagram indicates the process of the alternative splicing: Lastly, it is important to mention about the differences between the eukaryotic and prokaryotic transcription. In prokaryotes, we have a special term for the DNA which is called an operon. Operon carries the information for more than one gene and transcribed by the single promoter, eventually create a single mRNA which contain more than one gene. This single mRNA will be translated and eventually produce more than one protein, this characteristic of the prokaryotic DNA is called as â€Å"polycistronic† gene. However, in eucaryotes, there is only one gene that is transcribed at once. That’s why eukaryotic organisms are called as â€Å"monocistronic†. Prokaryotic transcription is only dependent to one factor in order to hold the RNA polymerase on the DNA while the eukaryotic transcription needs so many transcription factor, additional proteins and mediators. Also, since the prokaryotic DNA is found in a loose conformation in its original form, there is no need to use additional modification to destroy the packed structure as in the eukaryotes. So prokaryotic transcription machinery doesn’t use any chromatin remodelling complex or histone modifications. Prokaryotes don’t have intron in their pre-mRNA so there is no splicing in their mRNA after it is synthesised. They are free from introns, their mRNA is composed of more than one gene. Lastly, prokaryotes don’t need additional cap modification after the transcription of the mRNA. the mRNA can be easily transcribed as soon as they are synthesised. References: Belfort, M. (1989). RNA: Catalysis, splicing, evolution. Amsterdam: Elsevier . Belotserkovskaya, R., Saunders, A., Lis, J., Reinberg, D. (n.d.). Transcription through chromatin: Understanding a complex FACT. Biochimica Et Biophysica Acta (BBA) Gene Structure and Expression, 87-99. Dahlberg, J. (1989). RNA processing. San Diego: Academic Press. Darnell, J., Lodish, H. (1990). Molecular cell biology (2nd ed.). New York: Scientific American Books :. Elliott, D., Ladomery, M. (2011). Molecular biology of RNA. Oxford: Oxford University Press. Goodrich, J. A.; Tjian, R. (1994). Transcription factors IIE and IIH and ATP hydrolysis direct promoter clearance by RNA polymerase II. Cell. Latchman, D. (2004). Eukaryotic transcription factors (4th ed.). Amsterdam: Elsevier/Academic Press. Locker, J. (2001). Transcription factors. Oxford: BIOS ;. Ohyama, T. (2005). DNA conformation and transcription. Georgetown, Tex.: Landes Bioscience/Eurekah.com ;. Raven, Peter H. (2011). Biology (9th ed.). New York: McGraw-Hill. Stamm, S. (2012). Alternative pre-mRNA splicing theory and protocols. Weinheim: Wiley-Blackwell. Wagner, R. (2000). Transcription regulation in prokaryotes. Oxford: Oxford University Press.

Suicide :: essays research papers fc

I will never forget what happened in my high school merely five months ago. Chuck was 16 years old, a junior in high school, and a star football player. In December of 2002, he began to have many troubles in his life. His girlfriend of about one year broke off their relationship, and declined his invitation to the school’s annual Christmas Ball. In the days leading up to this dance, Chuck had lost his after-school job, and had several conferences with his guidance counselor about his poor academic performance. On top of all of these factors, Chuck went to a car dealership to put a down payment a car he had his heart set on, and discovered it had already been sold. Chuck went to the Christmas Ball alone, following his ex-girlfriend around for the majority of the night. Midway through the evening, one of Chuck’s classmates screamed at him, â€Å"She doesn’t like you, get away from her and get a life!† Chuck spent the rest of the evening sitting alone. He di d not attend any of the after-parties that evening, and the next day, Chuck’s parents discovered his body hanging in their shower. Have you ever felt like just throwing it all away? No matter how pleasant a person’s life is, there are often times where a person feels overpowered by feelings of doubt, despair, and hopelessness. The majorities of people either push these feelings aside or cope with them, and the feelings diminish and disappear. For others, these emotions never seem to fade away; intense negative feelings continually overwhelm their lives and lead them to believe there is no way to escape or cope with the problems at hand. They turn to suicide, seeing death as the only solution to their problems. Suicide is a problem that affects all people, in every age group, from every background, in every social class, and should not be taken lightly. Due to the graveness of the issue and its detrimental effects, all people are socially obligated to help prevent suicide at all costs. Suicide is defined as â€Å"the act or an instance of intentionally killing oneself.† (www.dictionary.com) In the United States alone, an average of 29,350 people commits suicide each year, equaling approximately 80 people a day, one person every 18 minutes. Suicide is the 11th leading cause of death in the United States. Although the overall rate of suicide has dropped in recent years, teen suicide has increased, becoming the 3rd leading cause of death for individuals between the ages of 15 and 24.

Distress in The Love Song of J.Alfred Prufrock Essay -- Love Song J. A

The Love Song of J.Alfred Prufrock - The Distress of J.Alfred Prufrock The human psyche is divided into three distinct aspects: the Persona, the Shadow, and the Anima/Animus; at least, it is according to Jungian Psychology. Drawing heavily on the theories developed by Freud, Jung's psychological concepts tell us that if these three facets are not properly integrated - that is, if one of the three is overly dominant, or repressed, or all three are in conflict with each other - then an individual's energies - his libido - will be out of alignment, causing psychological distress and unconscious problems. The Love Song of J.Alfred Prufrock, if read Archetypaly, reveals to us such an individual. J.Alfred Prufrock, the nebbish little man that he is, has some very serious problems - he is extremely indecisive, obsessed with trivial details, and frets over inconsequentials ('Shall I part my hair behind? Do I dare eat a peach?'); more importantly, he seems to have an inability to confront the opposite sex, choosing instead to develop elaborate fantasies in which to meet imaginary women - all of whom seem to be either cruel, vain, or sinister in some subtle way. An archetypal analysis drawing upon Jung's theories seeks to uncover the reasons behind Prufrock's neurosis. The first line of the poem - 'Let us go then, you and I' - gives an immediate insight into Prufrock's problem: his psyche is out of joint. The 'You' and 'I' of the poem are two aspects of his personality: his Shadow and his Persona, respectively. Prufrock is very much aware of the schism within his own mind. His Persona - the aspect of himself he presents to the social world - remains dominant most of the time. His Shadow, however, comp... ...s earlier illness. Unfortunately for Prufrock, this apparent cycle is entirely contained within a small portion of the whole. Prufrock participates in a greater cycle, but one that seems to end, not in life, or rebirth, but in a symbolic death. He is a crab, a '... pair of ragged claws/Scuttling across the floors of silent seas', a man who wakes... and drowns. His cycle ends negatively, without the rebirth. He never gets his act together, never integrates his personality properly, never stops being the sad little nebbish of a man that he is; the cracks in Prufrock are never mended. -Michael J.Noakes Works Cited Eliot, T.S. "The Love Song of J.Alfred Prufrock ." The Norton Anthology of English Literature, Volume Two. Ed. M.H.Abrams, 6th Edition. New York: Norton, 1993. 2140-2143. Distress in The Love Song of J.Alfred Prufrock Essay -- Love Song J. A The Love Song of J.Alfred Prufrock - The Distress of J.Alfred Prufrock The human psyche is divided into three distinct aspects: the Persona, the Shadow, and the Anima/Animus; at least, it is according to Jungian Psychology. Drawing heavily on the theories developed by Freud, Jung's psychological concepts tell us that if these three facets are not properly integrated - that is, if one of the three is overly dominant, or repressed, or all three are in conflict with each other - then an individual's energies - his libido - will be out of alignment, causing psychological distress and unconscious problems. The Love Song of J.Alfred Prufrock, if read Archetypaly, reveals to us such an individual. J.Alfred Prufrock, the nebbish little man that he is, has some very serious problems - he is extremely indecisive, obsessed with trivial details, and frets over inconsequentials ('Shall I part my hair behind? Do I dare eat a peach?'); more importantly, he seems to have an inability to confront the opposite sex, choosing instead to develop elaborate fantasies in which to meet imaginary women - all of whom seem to be either cruel, vain, or sinister in some subtle way. An archetypal analysis drawing upon Jung's theories seeks to uncover the reasons behind Prufrock's neurosis. The first line of the poem - 'Let us go then, you and I' - gives an immediate insight into Prufrock's problem: his psyche is out of joint. The 'You' and 'I' of the poem are two aspects of his personality: his Shadow and his Persona, respectively. Prufrock is very much aware of the schism within his own mind. His Persona - the aspect of himself he presents to the social world - remains dominant most of the time. His Shadow, however, comp... ...s earlier illness. Unfortunately for Prufrock, this apparent cycle is entirely contained within a small portion of the whole. Prufrock participates in a greater cycle, but one that seems to end, not in life, or rebirth, but in a symbolic death. He is a crab, a '... pair of ragged claws/Scuttling across the floors of silent seas', a man who wakes... and drowns. His cycle ends negatively, without the rebirth. He never gets his act together, never integrates his personality properly, never stops being the sad little nebbish of a man that he is; the cracks in Prufrock are never mended. -Michael J.Noakes Works Cited Eliot, T.S. "The Love Song of J.Alfred Prufrock ." The Norton Anthology of English Literature, Volume Two. Ed. M.H.Abrams, 6th Edition. New York: Norton, 1993. 2140-2143.

Post Traumatic Stress Disorder and Veterans Essay -- Post-traumatic st

Post Traumatic Stress Disorder (PTSD), originally associated with combat, has always been around in some shape or form but it was not until 1980 that it was named Post Traumatic Stress Disorder and became an accredited diagnosis (Rothschild). The fact is PTSD is one of many names for an old problem; that war has always had a severe psychological impact on people in immediate and lasting ways. PTSD has a history that is as long and significant as the world’s war history - thousands of years. Although, the diagnosis has not been around for that long, different names and symptoms of PTSD always have been. Some physical symptoms include increased blood pressure, excessive heart rate, rapid breathing, muscle tension, nausea, diarrhea, problems with vision, speech, walking disorders, convulsive vomiting, cardiac palpitations, twitching or spasms, weakness and severe muscular cramps. The individual may also suffer from psychological symptoms, such as violent nightmares, flashbacks , melancholy, disturbed sleep or insomnia, loss of appetite, and anxieties when certain things remind them such as the anniversary date of the event (Peterson, 2009). Examples of PTSD can be found as early as in ancient Greek and Roman history, for example the Greek historian Herodotus mentions â€Å"an Athenian warrior who went permanently blind when the soldier standing next to him was killed, although the blinded soldier was wounded in no part of his body† when he wrote about the battle of Marathon in 490 B.C. He â€Å"also writes of the Spartan commander Leonidas, who, at the battle of Thermopylae Pass in 480 B.C., dismissed his men from joining the combat because he clearly recognized they were psychologically spent from previous battles.-They had no heart for ... ...to gain economically (combined with veterans’ pride and distrust) accounts for the fact that of 830,000 Vietnam veterans with full-blown or partial PTSD, only 55,119 have filed claims, and the medical boards have only believed 28,411 (Triangle Institute study, July 1990) (Bentley, 2005)! Works Cited Bentley, S. (2005, March). Short History of PTSD. Retrieved 12 6, 2011, from Veteran: http://www.vva.org/archive/TheVeteran/2005_03/feature_HistoryPTSD.htm PBS. (n.d.). PBS Org. Retrieved 12 6, 2011, from The Soldier's Heart: http://www.pbs.org/wgbh/pages/frontline/shows/heart/themes/shellshock.html Peterson, D. (2009). From Shell shock to PTSD. Retrieved 12 6, 2011, from http://www.las.illinois.edu/news/2009/ptsd/ Rothschild, B. (n.d.). Post-Traumatic Stress Disorder:. Retrieved 11 6, 20011, from http://www.healing-arts.org/tir/n-r-rothschild.htm

Charles Chocolate case Essay

Traelene beals â€Å"9am Friday† First, a formal case analysis – This should be written in a business style format using short paragraphs and sentences, self-explanatory headings, and any figures/tables that would facilitate reading. Reports are to be no longer than 2000 words (excluding appendices Issues to be addressed – No measures of productivity or efficiaency in the plant- no way of telling if the plant was doing a good job. Difficult demand forcasting due to seasonality of sales. 2 new stores had ok type sales. Best clients of Wholesale sales changed focus on cheaper items and also own products. Sales agents could be fired with 90 days notice. Other parts of US haven’t heard of us. Of the 221 wholesale customers purchase just $1000 worth of products. Other 125 purchase between 1000 and 2000 per year. Sales agents, don’t know how to represent the all products. Speculating future orders from wholesale clients to customize boxes and logos-waste of capital. 60% of the total online/phone orders were from existing customers. Online sales haven’t grown – orders processed within 3-4 days. Why ship internationaly(only 5%)—Antartica Really Summer problem at Sandwich heaven – why did staff leave? How to get new staff in this tight labour market. Marketing service – old fashioned — Undefined Target market? Packaging ? (tourists publicatins, seasonal print media and radio spots) How to increase awareness without diluting the brand. Leverage on solid search engine raankings to promote online sales Basic website, reminder service to customers-good/bad? Sales agents dint provide links to top accounts. Companies revenues had grown because of Sandwich heaven(franchising) High reliability on tourism. Corporate gift marketing – 25% discount – market was good – Explore boston ? ice cream sales Increase retail penetration? Acquire a niche chocolate company? What about the tradiotnal brand name? Internal capacity? Relocate factory? Background Charles produced high-quality, hand wrapped chocolates, Portland creams. Best quality, many loyal customers across the world. Huge factory(24000 sq foot)-owned-. Only 75 retail and 35 production employees, 20 in management. Working hours 7am-4pm(each day). – Leverage long shelf life Wholesale production required early planning and online sales required late production†¦ Production planning was completed by data distortions arising from out-of stock and over stock issues. – out of stock-over production – killed the pricing – by discount pricing. Special orders put the whole order on a stand still Retail stores provided 50% of sales. – Wholesale had 30% of sales – Charles is just used as an add on product. Online and phone – online 4% of total sales. –phone is 6% of total sales—60% of all these orders were from regular orders.. Avg sales $138 by phone and $91 from website†¦High growth industry†¦ Sandwich heaven – 10% of sales – Industry High demand for organic/dark chocolate- anti oxidant properties.

Economic Development of India Essay

The capital market is the market for securities, where Companies and governments can raise long-term funds. It is a market in which money is lent for periods longer than a year. A nation’s capital market includes such financial institutions as banks, insurance companies, and stock exchanges that channel long-term investment funds to commercial and industrial borrowers. Unlike the money market, on which lending is ordinarily short term, the capital market typically finances fixed investments like those in buildings and machinery. Nature and Constituents: The capital market consists of number of individuals and institutions (including the government) that canalize the supply and demand for longterm capital and claims on capital. The stock exchange, commercial banks, co-operative banks, saving banks, development banks, insurance companies, investment trust or companies, etc., are important constituents of the capital markets. The capital market, like the money market, has three important Components, namely the suppliers of loanable funds, the borrowers and the Intermediaries who deal with the leaders on the one hand and the Borrowers on the other. ï  ® The demand for capital comes mostly from agriculture, industry, trade The government. The predominant form of industrial organization developed Capital Market becomes a necessary infrastructure for fast industrialization,and hence its important for the economy because india is a land if agriculture where more than 70 % of population depends upon agriculture and as India is also an developing nation so,industrialization is must necessary In this topic we have discussed that the development of stock market must contributes to economic growth both directly and indirectly. Hence stock market plays an important role in the economy of a country. Following the direct channel, we show that market liquidity has a positive impact on growth and indirectly market size affects investments which must affect growth of the country. Security markets also play a crucial role in economic growth and financial stability. The primary purpose of security markets is to serve as a mechanism for the transformation of savings into financing for the real sector, and hence constituting an alternative to bank financing. Recent local and global studies show that there is a positive correlation between the developments of stock markets and economic growth. Stock markets must be very efficient in the allocation of capital to its highest-value users. These markets also help to increase savings and investment, which are essential for economic development. If an equity market is informationally inefficient then investors face difficulties in choosing the optimal investment, because information on corporate performance is slow. Role of Securities Markets in Economic Development The increasing stringency of terms on both domestic and international loans, the urgency of mobilizing domestic resources by means other than dent finance has been greatly identified. The alternative to debt finance, of course is equity market. Capital market refers to the market for long and medium term funds for the business enterprise. It can be divided into securities and non-securities market. Securities market in turn may be divided unto the markets for primary issues and markets for secondary trading of the issued securities. In the secondary market, the existing securities change from the investor to another. There is no additional flow of funds for investment purposes in a secondary market; it only provides liquidity and marketability to the existing securities. A secondary market is very essential for a new issue market to develop. The secondary market can play most crucial functions in the pace of economic development by the promotion of savings and investment and efficient allocation of finds among the users. The securities market offers both investors and issues a broad spectrum of investment alternatives, which can help increase the level of both savings and investment. An efficient capital market can play the crucial role in mobilizing domestic savings for the purpose of investment

Ip Camera

Basic – analog and digital Branded ones – Bosch, AXIS, Mobotix Biggest Taiwan brand Vivotek Check out AXIS and Mobotix trend in each country. Bosch is following up with AXIS and Mobotix. Bosch website is good in technical. Vivotek is good in hardware. Bosch and Airlive software are good. Analog camera has BNC connector (round connector). Can convert analog to digital? Yes is possible. Cost is 25 USD to 250USD. Can convert to BNC to RJ45 or even to Fibre. Lens affect the viewing angle from 4 MM, 6MM, 8MM, 16MM. Manufacturer is Sharp and Sony. Lens also got differentiate into CMOS and optical lens. Optical lens consume more bandwidth.AXIS has the best CMOS lens and they can make it closer to optical lens. MTBF- Mean time before failures. Our AP is 200,000HRS which is quite safe. Camera also have MTBF timing. Bosch have best datasheet. Digital cameras is using RJ45, LAN cable. Digital have both indoor and outdoor. DVR for analog have BNC connector. 16 holes. 1 hole 1 chann el. DVR is for digital camera. DVR is mostly for soho use up to 16 channels or even 32 channels = 32 digital cameras. 1 camera 1 ip address. DVR – we have 1 setup box and 1 harddisk and few buttons for control. Older form of DVR is onsite recording. 2nd generation is with Lan cables so is going via IP.Can record offsite. PTZ cameras – Pan Tilt Zoom.. which the lens is optical. NVR – network video recording is like a NAS, up to dual CPU power with single GIGBABIT port for connecting high resolution cameras for processing. Can connect up to 254 cameras to 1 NVR (255 ip address for Class C subnet mask). Codec is required for converting the recording for onsite. H. 264 and MPEG file version. For future, people are doing bigger and larger and scalable NVR to increase the number of cameras. Raid is now up to 0 and 1. Next year will be raid 5 and raid 6. inside have 4 CPU inside 4U CPU rack with 2 harddisk with redundant power. Software –Questions to ask. 1) Wha t is the FRAME per second? FPS. Recording for IP camera is 29FPS is good (for wired). Wireless camera is normally 3 to 5 FPS for viewing. Recording is still 29FPS for wireless. 2) Resolutions. 320 x 320, 480 x 320, 640 x 480, 1024 x 768 etc. higher resolution will consume higher bandwidth. For analog is defined as lines. Eg HD TV has 1080 lines. 3)CODEC file type. For night vision is based on lux. 0. 5 LUX is able to see already. 1 LUX – 40W of light bulbs. IP cameras do not take standard POE but it take POE+. AXIS – strong at indoor Mobotix – strong at outdoor Vivotek – Outdoor and indoor Bosch – Outdoor